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Western blot analysis of extracts of various cell lines, using CD13/CD13/ANPEP antibody (A5662) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
CD13/ANPEP Rabbit pAb
Catalog No：AB-A5662
Manufacturer：ABclonal
Synonyms：ANPEP;APN;CD13;GP150;LAP1;P150;PEPN
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CD13/ANPEP Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 668-967 of human CD13/CD13/ANPEP (NP_001141.2). |
| Gene ID: | 290 |
| Swiss prot: | P15144 |
| Molecular weight: | 109kDa |
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