Western blot analysis of extracts of various cell lines, using CD68 antibody (A5515) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
CD68 Rabbit pAb
Catalog No:AB-A5515
Manufacturer:ABclonal
Synonyms:CD68;GP110;LAMP4;SCARD1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €295,00
Product Name: | CD68 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. |
Immunogen: | A synthetic peptide of human CD68 |
Gene ID: | 968 |
Swiss prot: | P34810 |
Molecular weight: | 31kDa/34kDa/37kDa |
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