Western blot analysis of extracts of various cell lines, using GARS antibody (A5435) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts of 293T cells, using GARS antibody (A5435) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
GARS Rabbit pAb
Catalog No:AB-A5435
Manufacturer:ABclonal
Synonyms:GARS;CMT2D;DSMAV;GlyRS;HMN5;SMAD1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GARS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2). |
Gene ID: | 2617 |
Swiss prot: | P41250 |
Molecular weight: | 83kDa |
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