Western blot analysis of extracts of various cell lines, using CUL4B antibody (A5400) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
CUL4B Rabbit pAb
Catalog No:AB-A5400
Manufacturer:ABclonal
Synonyms:CUL4B;CUL-4B;MRXHF2;MRXS15;MRXSC;SFM2;cullin-4B
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CUL4B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3). |
Gene ID: | 8450 |
Swiss prot: | Q13620 |
Molecular weight: | 84kDa/102kDa/103kDa |
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