Western blot analysis of extracts of various cell lines, using RAD17 antibody (A5359) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
RAD17 Rabbit pAb
Catalog No:AB-A5359
Manufacturer:ABclonal
Synonyms:RAD17;CCYC;HRAD17;R24L;RAD17SP;RAD24
Applications:WB,IF,IP
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RAD17 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1). |
Gene ID: | 5884 |
Swiss prot: | O75943 |
Molecular weight: | 57kDa/66kDa/75kDa/77kDa |
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