Western blot analysis of extracts of K-562 cells, using HADHA antibody (A5346) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (A5346) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] HADHA Rabbit pAb
Catalog No:AB-A5346
Manufacturer:ABclonal
Synonyms:HADHA;ECHA;GBP;HADH;LCEH;LCHAD;MTPA;TP-ALPHA
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] HADHA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2). |
Gene ID: | 3030 |
Swiss prot: | P40939 |
Molecular weight: | 28kDa/83kDa |
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