Western blot analysis of extracts of various cell lines, using GTF2A1 antibody (A5345) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
GTF2A1 Rabbit pAb
Catalog No:AB-A5345
Manufacturer:ABclonal
Synonyms:GTF2A1;TF2A1;TFIIA;TFIIA-42;TFIIAL
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GTF2A1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human GTF2A1 (NP_056943.1). |
Gene ID: | 2957 |
Swiss prot: | P52655 |
Molecular weight: | 37kDa/41kDa |
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