Western blot analysis of extracts of various cell lines, using GTF2H2 Antibody (A5317) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
GTF2H2 Rabbit pAb
Catalog No:AB-A5317
Manufacturer:ABclonal
Synonyms:GTF2H2;BTF2;BTF2P44;T-BTF2P44;TFIIH;p44
Applications:WB,IHC
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GTF2H2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF2H2 (NP_001506.1). |
Gene ID: | 2966 |
Swiss prot: | Q13888 |
Molecular weight: | 44kDa |
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