Western blot analysis of extracts of various cell lines, using GMNN antibody (A5316) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
GMNN Rabbit pAb
Catalog No:AB-A5316
Manufacturer:ABclonal
Synonyms:GMNN;Gem;MGORS6;geminin
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GMNN Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1). |
Gene ID: | 51053 |
Swiss prot: | O75496 |
Molecular weight: | 23kDa |
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