Western blot analysis of extracts of NCI-H460 cells, using Brachyury Rabbit mAb (A5078) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Brachyury Rabbit mAb (A5078) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Brachyury Rabbit mAb
Catalog No:AB-A5078
Manufacturer:ABclonal
Synonyms:SAVA, TFT
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Brachyury Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
Immunogen: | A synthesized peptide derived from human Brachyury |
Gene ID: | 6862 |
Swiss prot: | O15178 |
Molecular weight: | 47,41kDa |
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