Western blot analysis of extracts of various cell lines, using UPF1/RENT1 Rabbit mAb (A5071) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
UPF1/RENT1 Rabbit mAb
Catalog No:AB-A5071
Manufacturer:ABclonal
Synonyms:HUPF1, NORF1, RENT1, pNORF1, smg-2
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | UPF1/RENT1 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Immunogen: | A synthesized peptide derived from human UPF1/RENT1 |
Gene ID: | 5976 |
Swiss prot: | Q92900 |
Molecular weight: | 130kDa |
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