Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Hydroxyl-Histone H2A-Y39 Rabbit mAb
Catalog No:AB-A4827
Manufacturer:ABclonal
Synonyms:H2A.1, H2A.2, H2A/a, H2AFA
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Hydroxyl-Histone H2A-Y39 Rabbit mAb |
Category: | Other Modified Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
Immunogen: | A hydroxyl specific peptide corresponding to residues surrounding Y39 of Histone H2A |
Gene ID: | 3012 |
Swiss prot: | P04908 |
Molecular weight: | 14kDa |
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