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Western blot analysis of extracts of various cell lines, using BAG3 Rabbit mAb (A4720) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse kidney using BAG3 Rabbit mAb (A4720) at dilution of 1:100 (40x lens).
BAG3 Rabbit mAb
Catalog No：AB-A4720
Manufacturer：ABclonal
Synonyms：BAG-3, BIS, CAIR-1, MFM6
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | BAG3 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human BAG3 |
| Gene ID: | 9531 |
| Swiss prot: | O95817 |
| Molecular weight: | 80kDa |
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