Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2/U2AF65 Rabbit mAb (A4552) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2/U2AF65 Rabbit mAb (A4552) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
U2AF2/U2AF65 Rabbit mAb
Catalog No:AB-A4552
Manufacturer:ABclonal
Synonyms:U2AF65
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | U2AF2/U2AF65 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human U2AF2/U2AF2/U2AF65 |
Gene ID: | 11338 |
Swiss prot: | P26368 |
Molecular weight: | 54kDa |
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