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Western blot analysis of extracts of various cell lines, using AMPKβ1 Rabbit mAb (A4344) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts of Mouse brain, using AMPKβ1 Rabbit mAb (A4344) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded rat ovary using AMPKβ1 Rabbit mAb (A4344) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using AMPKβ1 Rabbit mAb (A4344) at dilution of 1:100 (40x lens).
AMPKβ1 Rabbit mAb
Catalog No：AB-A4344
Manufacturer：ABclonal
Synonyms：AMPK, HAMPKb
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | AMPK尾1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human AMPK尾1 |
| Gene ID: | 5564 |
| Swiss prot: | Q9Y478 |
| Molecular weight: | 38kDa |
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