Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody (A4274) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 antibody (A4274) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] ARHGEF1 Rabbit pAb
Catalog No:AB-A4274
Manufacturer:ABclonal
Synonyms:ARHGEF1;GEF1;LBCL2;LSC;P115-RHOGEF;SUB1.5
Applications:WB,IHC,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] ARHGEF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2). |
Gene ID: | 9138 |
Swiss prot: | Q92888 |
Molecular weight: | 98kDa/102kDa/104kDa/105kDa |
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