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Western blot analysis of extracts of various cell lines, using P4HA2 antibody (A4262) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of Rat placenta, using P4HA2 antibody (A4262) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
P4HA2 Rabbit pAb
Catalog No：AB-A4262
Manufacturer：ABclonal
Synonyms：P4HA2;MYP25
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | P4HA2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-533 of human P4HA2 (NP_001136070.1). |
| Gene ID: | 8974 |
| Swiss prot: | O15460 |
| Molecular weight: | 60kDa |
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