Western blot analysis of extracts of various cell lines, using IARS antibody (A10190) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
IARS Rabbit pAb
Catalog No:AB-A10190
Manufacturer:ABclonal
Synonyms:IARS;GRIDHH;IARS1;ILERS;ILRS;IRS;PRO0785
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | IARS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2). |
Gene ID: | 3376 |
Swiss prot: | P41252 |
Molecular weight: | 144kDa |
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