Western blot analysis of extracts of various cell lines, using ALDOB antibody (A3728) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
ALDOB Rabbit pAb
Catalog No:AB-A3728
Manufacturer:ABclonal
Synonyms:ALDOB;ALDB;ALDO2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ALDOB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related ‘housekeeping’ genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALDOB (NP_000026.2). |
Gene ID: | 229 |
Swiss prot: | P05062 |
Molecular weight: | 39kDa |
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