Western blot analysis of extracts of HeLa cells, using CSPG4 antibody (A3592) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (A3592) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] CSPG4 Rabbit pAb
Catalog No:AB-A3592
Manufacturer:ABclonal
Synonyms:CSPG4;HMW-MAA;MCSP;MCSPG;MEL-CSPG;MSK16;NG2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] CSPG4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2). |
Gene ID: | 1464 |
Swiss prot: | Q6UVK1 |
Molecular weight: | 250kDa |
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