Western blot analysis of extracts of various cell lines, using MEK3 Rabbit mAb (A3263) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
MEK3 Rabbit mAb
Catalog No:AB-A3263
Manufacturer:ABclonal
Synonyms:MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | MEK3 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human MEK3 |
Gene ID: | 5606 |
Swiss prot: | P46734 |
Molecular weight: | 39kDa |
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