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Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R26 antibody (A3153) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Symmetric DiMethyl-Histone H3-R26 Rabbit pAb
Catalog No：AB-A3153
Manufacturer：ABclonal
Synonyms：HIST1H3J;H3/j;H3FJ
Applications：WB,IF
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Symmetric DiMethyl-Histone H3-R26 Rabbit pAb |
| Category: | Methylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3 |
| Gene ID: | 8356 |
| Swiss prot: | P68431 |
| Molecular weight: | 15kDa |
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