Western blot analysis of extracts of various cell lines, using MYSM1 antibody (A3102) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
MYSM1 Rabbit pAb
Catalog No:AB-A3102
Manufacturer:ABclonal
Synonyms:MYSM1;2A-DUB;2ADUB
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MYSM1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human MYSM1 (NP_001078956.1). |
Gene ID: | 114803 |
Swiss prot: | Q5VVJ2 |
Molecular weight: | 26kDa/66kDa/95kDa |
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