Western blot analysis of extracts of various cell lines, using AKAP8 antibody (A3049) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
AKAP8 Rabbit pAb
Catalog No:AB-A3049
Manufacturer:ABclonal
Synonyms:AKAP8;AKAP 95;AKAP-8;AKAP-95;AKAP95
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | AKAP8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1). |
Gene ID: | 10270 |
Swiss prot: | O43823 |
Molecular weight: | 76kDa |
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