Western blot analysis of extracts of various cell lines, using B-Raf antibody (A2988) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
B-Raf Rabbit pAb
Catalog No:AB-A2988
Manufacturer:ABclonal
Synonyms:BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | B-Raf Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
Immunogen: | A synthetic peptide of human B-Raf |
Gene ID: | 673 |
Swiss prot: | P15056 |
Molecular weight: | 84kDa |
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