Western blot analysis of extracts of various cell lines, using SFRP1 antibody (A2911) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
SFRP1 Rabbit pAb
Catalog No:AB-A2911
Manufacturer:ABclonal
Synonyms:SFRP1;FRP;FRP-1;FRP1;FrzA;SARP2
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SFRP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-314 of human SFRP1 (NP_003003.3). |
Gene ID: | 6422 |
Swiss prot: | Q8N474 |
Molecular weight: | 35kDa |
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