Western blot analysis of extracts of various cell lines, using DNMT3B antibody (A2899) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
DNMT3B Rabbit pAb
Catalog No:AB-A2899
Manufacturer:ABclonal
Synonyms:DNMT3B;ICF;ICF1;M.HsaIIIB
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DNMT3B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. |
Immunogen: | A synthetic peptide of human DNMT3B |
Gene ID: | 1789 |
Swiss prot: | Q9UBC3 |
Molecular weight: | 77-95kDa |
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