Western blot analysis of extracts of various cell lines, using GPT antibody (A2814) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
GPT Rabbit pAb
Catalog No:AB-A2814
Manufacturer:ABclonal
Synonyms:GPT;AAT1;ALT1;GPT1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GPT Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1). |
Gene ID: | 2875 |
Swiss prot: | P24298 |
Molecular weight: | 54kDa |
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