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Western blot analysis of extracts of various cell lines, using ACHE antibody (A2806) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse brain using ACHE antibody (A2806) at dilution of 1:100 (40x lens).
ACHE Rabbit pAb
Catalog No：AB-A2806
Manufacturer：ABclonal
Synonyms：ACHE;ACEE;ARACHE;N-ACHE;YT
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ACHE Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 398-617 of human ACHE (NP_056646.1). |
| Gene ID: | 43 |
| Swiss prot: | P22303 |
| Molecular weight: | 58kDa/65kDa/67kDa |
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