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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (A2636) at 1:300 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts of Jurkat cells, using Caspase-9 antibody (A2636) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human lung cancer using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-9 antibody (A2636) at dilution of 1:100 (40x lens).
Caspase-9 Rabbit pAb
Catalog No：AB-A2636
Manufacturer：ABclonal
Synonyms：CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Caspase-9 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant protein of human Caspase-9 |
| Gene ID: | 842 |
| Swiss prot: | P55211 |
| Molecular weight: | 17kDa/30kDa/36kDa/46kDa |
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