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Western blot analysis of extracts of various cell lines, using PDCD4 antibody (A2570) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (A2570) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] PDCD4 Rabbit pAb (A2570) at dilution of 1:25 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] PDCD4 Rabbit pAb (A2570) at dilution of 1:25 (40x lens).
[KO Validated] PDCD4 Rabbit pAb
Catalog No：AB-A2570
Manufacturer：ABclonal
Synonyms：PDCD4;H731
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] PDCD4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2). |
| Gene ID: | 27250 |
| Swiss prot: | Q53EL6 |
| Molecular weight: | 50kDa/51kDa |
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