Western blot analysis of extracts of various cell lines, using SKP1 antibody (A2566) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
SKP1 Rabbit pAb
Catalog No:AB-A2566
Manufacturer:ABclonal
Synonyms:SKP1;EMC19;OCP-II;OCP2;SKP1A;TCEB1L;p19A
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SKP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human SKP1 (NP_733779.1). |
Gene ID: | 6500 |
Swiss prot: | P63208 |
Molecular weight: | 18kDa |
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