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Western blot analysis of extracts of MCF7 cells, using [KO Validated] HMGB1 Rabbit pAb (A2553) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts from normal (control) and [KO Validated] HMGB1 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] HMGB1 Rabbit pAb antibody (A2553) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
[KO Validated] HMGB1 Rabbit pAb
Catalog No：AB-A2553
Manufacturer：ABclonal
Synonyms：HMG-1;HMG1;HMG3;SBP-1;HMGB1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HMGB1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1). |
| Gene ID: | 3146 |
| Swiss prot: | P09429 |
| Molecular weight: | 24kDa |
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