Western blot analysis of extracts of various cell lines, using Estrogen Receptor beta Rabbit pAb (A2546) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Estrogen Receptor beta Rabbit pAb
Catalog No:AB-A2546
Manufacturer:ABclonal
Synonyms:ESR2;ER-BETA;ESR-BETA;ESRB;ESTRB;Erb;NR3A2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Estrogen Receptor beta Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Estrogen Receptor beta (NP_001201832.1). |
Gene ID: | 2100 |
Swiss prot: | Q92731 |
Molecular weight: | 35-59kDa |
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