Western blot analysis of extracts of HeLa cells, using c-Fos antibody (A2444) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
c-Fos Rabbit pAb
Catalog No:AB-A2444
Manufacturer:ABclonal
Synonyms:FOS;AP-1;C-FOS;p55;c-Fos
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | c-Fos Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 90-190 of human c-Fos (NP_005243.1). |
Gene ID: | 2353 |
Swiss prot: | P01100 |
Molecular weight: | 28kDa/36kDa/40kDa |
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