Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H4-K20 antibody (A2371) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
DiMethyl-Histone H4-K20 Rabbit pAb
Catalog No:AB-A2371
Manufacturer:ABclonal
Synonyms:FO108;H4;H4/n;H4F2;H4FN;HIST2H4;Histone H4;HIST1H4A;HIST2H4A
Applications:WB,IHC,IF,IP,ChIP,ChIP-seq
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | DiMethyl-Histone H4-K20 Rabbit pAb |
Category: | Methylated Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. |
Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4 |
Gene ID: | 8370 |
Swiss prot: | P62805 |
Molecular weight: | 11kDa |
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