Western blot analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
TriMethyl-Histone H3-K27 Rabbit pAb
Catalog No:AB-A2363
Manufacturer:ABclonal
Synonyms:H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications:WB,IHC,IF,IP,ChIP,ChIP-seq
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €900,00
Product Name: | TriMethyl-Histone H3-K27 Rabbit pAb |
Category: | Methylated Antibodies |
Description: | Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling. |
Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
Gene ID: | 8290 |
Swiss prot: | Q16695 |
Molecular weight: | 15kDa |
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Catalog No:AB-A18824
Manufacturer:ABclonal
Synonyms:U2AF1; FP793; RN; RNU2AF1; U2AF35; U2AFBP; splicing factor U2AF 35 kDa subunit
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€110,00 – €410,00
Catalog No:AB-A18823
Manufacturer:ABclonal
Synonyms:TRIM28; KAP1; PPP1R157; RNF96; TF1B; TIF1B; tripartite motif containing 28
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€110,00 – €410,00
Catalog No:AB-A18816
Manufacturer:ABclonal
Synonyms:HNRNPK; AUKS; CSBP; HNRPK; TUNP; heterogeneous nuclear ribonucleoprotein K
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€110,00 – €410,00
Catalog No:AB-A18651
Manufacturer:ABclonal
Synonyms:YAP1; COB1; YAP; YAP2; YAP65; YKI; Yes associated protein 1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-A18261
Manufacturer:ABclonal
Applications:WB
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €540,00
Catalog No:AB-A16199
Manufacturer:ABclonal
Synonyms:H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications:WB,IHC,IF,IP,ChIP
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Mouse
Conjugate:No
€65,00 – €525,00