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Western blot analysis of extracts of various cell lines, using CD86 antibody (A2353) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 300s.
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Immunohistochemistry of paraffin-embedded human tonsil using CD86 antibody (A2353) at dilution of 1:100 (40x lens).
CD86 Rabbit pAb
Catalog No：AB-A2353
Manufacturer：ABclonal
Synonyms：B7-2;B7.2;B70;CD28LG2;LAB72;CD86
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CD86 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4). |
| Gene ID: | 942 |
| Swiss prot: | P42081 |
| Molecular weight: | 12kDa/24kDa/28kDa/31kDa/37kDa |
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