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Western blot analysis of extracts of various cell lines, using HP1 gamma/HP1 gamma/CBX3 antibody (A2248) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Western blot analysis of extracts from normal (control) and HP1 gamma/HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/HP1 gamma/CBX3 antibody (A2248) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] HP1 gamma/HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] HP1 gamma/HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] HP1 gamma/HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:50 (40x lens).
[KO Validated] HP1 gamma/CBX3 Rabbit pAb
Catalog No：AB-A2248
Manufacturer：ABclonal
Synonyms：CBX3;HECH;HP1-GAMMA;HP1Hs-gamma
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HP1 gamma/CBX3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5′ UTR, have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human HP1 gamma/HP1 gamma/CBX3 (NP_009207.2). |
| Gene ID: | 11335 |
| Swiss prot: | Q13185 |
| Molecular weight: | 20kDa |
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