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Western blot analysis of extracts of various cell lines, using CHD3 antibody (A2221) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
CHD3 Rabbit pAb
Catalog No：AB-A2221
Manufacturer：ABclonal
Synonyms：CHD3;Mi-2a;Mi2-ALPHA;ZFH
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CHD3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human CHD3 (NP_001005273.1). |
| Gene ID: | 1107 |
| Swiss prot: | Q12873 |
| Molecular weight: | 222kDa/226kDa/233kDa |
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