Western blot analysis of extracts of various cell lines, using Claudin 1 antibody (A2196) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Claudin 1 Rabbit pAb
Catalog No:AB-A2196
Manufacturer:ABclonal
Synonyms:CLDN1;CLD1;ILVASC;SEMP1;claudin-1;Claudin 1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Claudin 1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Claudin 1 (NP_066924.1). |
Gene ID: | 9076 |
Swiss prot: | O95832 |
Molecular weight: | 22kDa |
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