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Western blot analysis of extracts of various cell lines, using ZAP70 antibody (A2195) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded human amygdalitis using ZAP70 antibody (A2195) at dilution of 1:100 (40x lens).
ZAP70 Rabbit pAb
Catalog No：AB-A2195
Manufacturer：ABclonal
Synonyms：ZAP70;ADMIO2;IMD48;SRK;STCD;STD;TZK;ZAP-70
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ZAP70 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ZAP70 (NP_001070.2). |
| Gene ID: | 7535 |
| Swiss prot: | P43403 |
| Molecular weight: | 35kDa/55kDa/69kDa |
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