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Western blot analysis of extracts of Mouse thymus, using LCK antibody (A2177) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using LCK antibody (A2177) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat spleen using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).
LCK Rabbit pAb
Catalog No：AB-A2177
Manufacturer：ABclonal
Synonyms：LCK;IMD22;LSK;YT16;p56lck;pp58lck
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | LCK Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LCK (NP_001036236.1). |
| Gene ID: | 3932 |
| Swiss prot: | P06239 |
| Molecular weight: | 40kDa/58kDa/61kDa |
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