Western blot analysis of extracts of various cell lines, using CYP2E1 antibody (A2160) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
CYP2E1 Rabbit pAb
Catalog No:AB-A2160
Manufacturer:ABclonal
Synonyms:CYP2E1;CPE1;CYP2E;P450-J;P450C2E
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CYP2E1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1). |
Gene ID: | 1571 |
Swiss prot: | P05181 |
Molecular weight: | 56kDa |
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