Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (A2136) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Cullin 1 Rabbit pAb
Catalog No:AB-A2136
Manufacturer:ABclonal
Synonyms:Cullin 1;CUL1;cullin-1;MGC149834;MGC149835
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cullin 1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1鈥3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-776 of human Cullin 1 (NP_003583.2). |
Gene ID: | 8454 |
Swiss prot: | Q13616 |
Molecular weight: | 89kDa |
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