Western blot analysis of extracts of various cell lines, using ERK5 antibody (A2111) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
ERK5 Rabbit pAb
Catalog No:AB-A2111
Manufacturer:ABclonal
Synonyms:MAPK7;BMK1;ERK4;ERK5;PRKM7
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ERK5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ERK5 (NP_002740.2). |
Gene ID: | 5598 |
Swiss prot: | Q13164 |
Molecular weight: | 50kDa/59kDa/73kDa/88kDa |
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