Western blot analysis of extracts of various cell lines, using PIAS1 antibody (A1007) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PIAS1 Rabbit pAb
Catalog No:AB-A1007
Manufacturer:ABclonal
Synonyms:PIAS1;DDXBP1;GBP;GU/RH-II;ZMIZ3
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PIAS1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 422-651 of human PIAS1 (NP_057250.1). |
Gene ID: | 8554 |
Swiss prot: | O75925 |
Molecular weight: | 36kDa/71kDa/72kDa |
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