Western blot analysis of extracts of various cell lines, using PATZ1 antibody (A10053) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
PATZ1 Rabbit pAb
Catalog No:AB-A10053
Manufacturer:ABclonal
Synonyms:PATZ1;MAZR;PATZ;RIAZ;ZBTB19;ZNF278;ZSG;dJ400N23
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PATZ1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human PATZ1 (NP_114440.1). |
Gene ID: | 23598 |
Swiss prot: | Q9HBE1 |
Molecular weight: | 57kDa/69kDa/74kDa |
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