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Western blot analysis of extracts of various cell lines, using TIGAR antibody (A20416) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunofluorescence analysis of NIH/3T3 cells using TIGAR Rabbit pAb (A20416) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using TIGAR Rabbit pAb (A20416) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using TIGAR Rabbit pAb (A20416) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
TIGAR Rabbit pAb
Catalog No：AB-A20416
Manufacturer：ABclonal
Synonyms：FR2BP; C12orf5
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€125,00 – €370,00
| Product Name: | TIGAR Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. |
| Immunogen: | Recombinant protein of human TIGAR. |
| Gene ID: | 57103 |
| Swiss prot: | Q9NQ88 |
| Molecular weight: | 30kDa |
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