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Western blot analysis of extracts of various cell lines, using POLR2A antibody (A20363) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
POLR2A Rabbit mAb
Catalog No：AB-A20363
Manufacturer：ABclonal
Synonyms：POLR2A;POLR2;POLRA;RPB1;RPBh1;RPO2;RPOL2;RpIILS;hRPB220;hsRPB1;Pol II
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€195,00 – €485,00
| Product Name: | POLR2A Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. |
| Immunogen: | Recombinant protein of human POLR2A. |
| Gene ID: | 5430 |
| Swiss prot: | P24928 |
| Molecular weight: | 63kDa/217kDa |
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